Jun 13, 2016
SDS-PAGE Cracking Buffer
- David Dunigan and Irina Agarkova1
- 1University of Nebraska-Lincoln
- VERVE Net
Protocol Citation: David Dunigan and Irina Agarkova 2016. SDS-PAGE Cracking Buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.erpbd5n
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: March 24, 2016
Last Modified: March 28, 2018
Protocol Integer ID: 2575
Abstract
Recipe to make 10ml of 2x stock
Guidelines
| 1M Tris-HCl pH 6.8 | 1.0 ml | |
| SDS | 0.4 g | |
| Glycerol | 2.0 ml | |
| 0.5 M EDTA | 0.5 ml | |
| Bromophenol Blue | 4 mg | |
| β-mercaptoethanol (14.7M) | 0.2 ml |
* It is better to add BME right before using buffer.