Jun 13, 2016
Running gel buffer (10X)
- DAVID DUNIGAN AND IRINA AGARKOVA
- VERVE Net
Protocol Citation: DAVID DUNIGAN AND IRINA AGARKOVA 2016. Running gel buffer (10X). protocols.io https://dx.doi.org/10.17504/protocols.io.ercbd2w
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: March 22, 2016
Last Modified: November 09, 2017
Protocol Integer ID: 2564
Abstract
Combine 45.5 gm of Tris base and 216.0 gm of Glycine.
Adjust the final volume to 1500 mL.
Note
The pH should be approximately 8.3 without adjustment.
SDS and 2-mercaptopropionic acid are added to the buffer prior to electrophoresis: the SDS to 0.1% and 1 mL of mercaptopropionic acid to 1 liter of 1X running buffer.