BioLegend’s Red Blood Cell (RBC) Lysis Buffer (Cat. No. 420301) has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions of hematopoietic tissues, such as spleen, as well as RBCs derived from peripheral blood. RBC Lysis Buffer contains ammonium chloride, which has been demonstrated to lyse RBCs with a minimum of harmful effects on lymphocytes