Feb 02, 2016
Pseudoalteromonas Media Recipes
- Matthew Sullivan1
- 1Matthew Sullivan Lab, University of Arizona, Ohio State University
- VERVE Net
- Sullivan Lab
Protocol Citation: Matthew Sullivan 2016. Pseudoalteromonas Media Recipes. protocols.io https://dx.doi.org/10.17504/protocols.io.de23gd
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: July 16, 2015
Last Modified: November 26, 2017
Protocol Integer ID: 1210
Guidelines
Zobell plates, 100% nutrient (1 L)
26 g sea salts
1 g yeast extract
5 g proteose peptone no. 2
fill to 1 L qH2O
12 g agar
Grow P.a. in PZM (=50% nutrient)
Zobell plates, 20% nutrient (1 L)
26 g sea salts
0.2 g yeast extract
1 g proteose peptone no. 2
fill to 1 L qH2O
12 g agar
Autoclave 1 L for 50 min., pour 10 ml/plate
Zobell liquid, (1 L)
26 g sea salts
1 g yeast extract
5 g proteose peptone no. 2
fill to 1 L qH2O
Top Zobell agar (; 1 L)
26 g sea salts
1 g yeast extract
5 g proteose peptone no. 2
fill to 1 L qH2O
6 g agar
MSM (1 L)
100 mM NaCl (5.8 g)
81.2 mM MgSO4·7H2O (20 g)
50 mM Tris base (3.02 g) OR 50 mL 1 M Tris (pH 7.6)
fill to 1 L qH2O
adjust to pH 7–8 if necessary
Artificial seawater, 26 psu (ASW; 1 L)
26 g sea salts
fill to 1 L qH2O