Combine:150mls Ethanol150mls distilled water3mls Glacial Acetic AcidSplit into 4 beakers (approx 75mls each) and add a stir bar.Beaker 1 - Add: 0.5g Acid Fuchsin and 1.0g Ponceau 2RBeaker 2 - Add: 0.45g Light green, SF, yellowishBeaker 3 - Add: 0.75g Orange G and 1.5g Phosphotungstic acidBeaker 4 - Add: 1.5g Phosphomolybdic acidWhen each reagent is maximally dissolved, combine the 4 dyes and filter. Cover storage bottle in tin foil to reduce light exposure. Remake each month.