The NEXTflex™ mtDNA-Seq Kit is designed to prepare single or paired-end DNA libraries of mitochondrial DNA (mtDNA) for sequencing using Illumina® platforms. The procedure isolates mitochondrial DNA from genomic DNA (gDNA) by selective digestion of linear nuclear DNA, followed by fragmentation of mtDNA and library preparation. Please see the full manual for additional details.(Note: This is the procedure for Cell Samples; for Blood Samples, see this protocol.)