Aug 10, 2016

Public workspaceLysis Buffer (10mM Tris-HCl, 2mM EDTA, 1% SDS)

DOI
dx.doi.org/10.17504/protocols.io.fjqbkmw
  • Sarah Hessen-Schmidt1
  • 1University of Southern California
Sarah Hessen-Schmidt
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DOI: dx.doi.org/10.17504/protocols.io.fjqbkmw
Protocol Citation: Sarah Hessen-Schmidt 2016. Lysis Buffer (10mM Tris-HCl, 2mM EDTA, 1% SDS). protocols.io https://dx.doi.org/10.17504/protocols.io.fjqbkmw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: August 10, 2016
Last Modified: March 27, 2018
Protocol Integer ID: 3408
Materials
STEP MATERIALS
ReagentSodium dodecyl sulfateMerck MilliporeSigma (Sigma-Aldrich)Catalog #436143-25G
ReagentSodium dodecyl sulfateMerck MilliporeSigma (Sigma-Aldrich)Catalog #436143-25G
Protocol materials
ReagentSodium dodecyl sulfateMerck MilliporeSigma (Sigma-Aldrich)Catalog #436143-25G
ReagentSodium dodecyl sulfateMerck MilliporeSigma (Sigma-Aldrich)Catalog #436143-25G
ReagentSodium dodecyl sulfateMerck MilliporeSigma (Sigma-Aldrich)Catalog #436143-25G
Add 2.5ml 1M Tris-HCl pH 7.5 to an autoclaved bottle
Protocol
1M Tris-HCl
NAME
1M Tris-HCl
CREATED BY
Sarah Hessen-Schmidt
Dissolve 30.275g Trizma base in 200ml MilliQ
ReagentTrizma BaseCatalog #93362
Adjust to pH 7.5 with HCl (~35ml)
Adjust total volume to 250ml with MilliQ
Autoclave
Add 1ml 0.5M EDTA
Protocol
0.5M EDTA
NAME
0.5M EDTA
CREATED BY
Sarah Hessen-Schmidt
46.525g EDTA disodium 2H20 in 200ml MilliQ
ReagentEthylenediaminetetraacetic acid disodium salt dihydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #E5134-1KG
Adjust to pH 8.0 with NaOH pellets (~5g)
Autoclave
Add 2.5g SDS
ReagentSodium dodecyl sulfateMerck MilliporeSigma (Sigma-Aldrich)Catalog #436143-25G
Adjust total volume to 250ml with MilliQ