Nov 03, 2014
IPTG – 1 M (100 x) Stock Solution
External link: http://store.p212121.com/IPTG/
Protocol Citation: Sean Seaver: IPTG – 1 M (100 x) Stock Solution. protocols.io https://dx.doi.org/10.17504/protocols.io.ci3ugm
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: October 23, 2014
Last Modified: March 28, 2018
Protocol Integer ID: 315
Abstract
How to make a IPTG – 1 M (100 x) Stock Solution
Guidelines
Materials
STEP MATERIALS
IPTGP212121Catalog #GB-I2481C
Syringe FilterP212121Catalog #LI-PES-0/22-13-S
Ampicillin Sodium SaltP212121Catalog #TWA-A-301
IPTGP212121Catalog #GB-I2481C
Syringe FilterP212121Catalog #LI-PES-0/22-13-S
Ampicillin Sodium SaltP212121Catalog #TWA-A-301
Protocol materials
Syringe FilterP212121Catalog #LI-PES-0/22-13-S
Ampicillin Sodium SaltP212121Catalog #TWA-A-301
IPTGP212121Catalog #GB-I2481C
Syringe FilterP212121Catalog #LI-PES-0/22-13-S
Ampicillin Sodium SaltP212121Catalog #TWA-A-301
IPTGP212121Catalog #GB-I2481C
IPTGP212121Catalog #GB-I2481C
Syringe FilterP212121Catalog #LI-PES-0/22-13-S
Ampicillin Sodium SaltP212121Catalog #TWA-A-301
Weigh 2.383 g of IPTG
2 g
IPTGP212121Catalog #GB-I2481C
Add 10 ml sterile H2O. Dissolve completely.
00:02:00
Prewet a 0.2 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water.
00:02:00
Syringe FilterP212121Catalog #LI-PES-0/22-13-S
Sterilize Ampicillin Stock through the prepared 0.2 µm syringe filter.
Store in 1 ml aliquots at -20°C for up to 1 year.
00:02:00
Ampicillin Sodium SaltP212121Catalog #TWA-A-301