Nov 03, 2014

Public workspaceHow to make a 25-50 mg/ml Chloramphenicol Stock Solution

DOI
dx.doi.org/10.17504/protocols.io.ci5ug5
Sean Seaver
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DOI: dx.doi.org/10.17504/protocols.io.ci5ug5
External link: http://store.p212121.com/chloramphenicol/
Protocol Citation: Sean Seaver: How to make a 25-50 mg/ml Chloramphenicol Stock Solution. protocols.io https://dx.doi.org/10.17504/protocols.io.ci5ug5
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: October 23, 2014
Last Modified: March 28, 2018
Protocol Integer ID: 317
Abstract
How to make a 25-50 mg/ml Chloramphenicol Stock Solution
Materials
MATERIALS
ReagentChloramphenicolP212121Catalog #GB-C-105
STEP MATERIALS
ReagentChloramphenicolP212121Catalog #GB-C-105
ReagentEthanolP212121Catalog #BE-BDH1156
ReagentChloramphenicolP212121Catalog #GB-C-105
ReagentEthanolP212121Catalog #BE-BDH1156
Protocol materials
ReagentChloramphenicolP212121Catalog #GB-C-105
ReagentChloramphenicolP212121Catalog #GB-C-105
ReagentEthanolP212121Catalog #BE-BDH1156
ReagentChloramphenicolP212121Catalog #GB-C-105
ReagentEthanolP212121Catalog #BE-BDH1156
ReagentChloramphenicolP212121Catalog #GB-C-105
ReagentEthanolP212121Catalog #BE-BDH1156
Weigh 0.25-0.5 g of Chloramphenicol
Duration00:04:00
ReagentChloramphenicolP212121Catalog #GB-C-105
Add 10 ml of 95% EtOH (70% may also be used).

Dissolve completely.

Stock may be kept at -20°C for 1 year.
Duration00:05:00
ReagentEthanolP212121Catalog #BE-BDH1156