Nov 03, 2014

Public workspaceHow to make a 1M DTT Stock Solution

DOI
dx.doi.org/10.17504/protocols.io.ci4ugv
Sean Seaver
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DOI: dx.doi.org/10.17504/protocols.io.ci4ugv
External link: http://store.p212121.com/DTT/
Protocol Citation: Sean Seaver: How to make a 1M DTT Stock Solution. protocols.io https://dx.doi.org/10.17504/protocols.io.ci4ugv
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: October 23, 2014
Last Modified: March 28, 2018
Protocol Integer ID: 316
Abstract
How to make a 1M DTT Stock Solution
Materials
STEP MATERIALS
ReagentDTTP212121Catalog #SV-DTT
ReagentSyringe FilterP212121Catalog #LI-PES-0/22-13-S
ReagentDTTP212121Catalog #SV-DTT
ReagentDTTP212121Catalog #SV-DTT
ReagentSyringe FilterP212121Catalog #LI-PES-0/22-13-S
ReagentDTTP212121Catalog #SV-DTT
Protocol materials
ReagentDTTP212121Catalog #SV-DTT
ReagentSyringe FilterP212121Catalog #LI-PES-0/22-13-S
ReagentDTTP212121Catalog #SV-DTT
ReagentDTTP212121Catalog #SV-DTT
ReagentSyringe FilterP212121Catalog #LI-PES-0/22-13-S
ReagentDTTP212121Catalog #SV-DTT
ReagentDTTP212121Catalog #SV-DTT
ReagentSyringe FilterP212121Catalog #LI-PES-0/22-13-S
ReagentDTTP212121Catalog #SV-DTT
Weigh 1.54 g of Dithiothreitol (DTT)
Amount2 g
Duration00:03:00
ReagentDTTP212121Catalog #SV-DTT
Add 10 ml of sterile dH2O. Dissolve completely.
Duration00:02:00
Prewet a 0.2 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water.
Duration00:02:00
ReagentSyringe FilterP212121Catalog #LI-PES-0/22-13-S
Sterilize DTT Stock through the prepared 0.2 µm syringe filter.

Aliquot into 2 ml tubes and store at -20°C. Stocks may be kept for 1 year.
Duration00:02:00
ReagentDTTP212121Catalog #SV-DTT