Nov 03, 2014
How to make a 1 M PMSF Stock Solution
External link: http://store.p212121.com/pmsf/
Protocol Citation: Sean Seaver: How to make a 1 M PMSF Stock Solution. protocols.io https://dx.doi.org/10.17504/protocols.io.ci8uhv
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: October 23, 2014
Last Modified: March 28, 2018
Protocol Integer ID: 320
Keywords: stock solution
Abstract
How to make a 1 M PMSF Stock Solution
Guidelines
PMSF is hygroscopic and is highly unstable in aqueous solutions. A typical working solution of PMSF is 1mM.
PMSF is an anti-protease! Do not allow to come into contact with skin.
PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25°C than at 4°C. The half-life of a 20mM aqueous solution of PMSF is ~35 minutes at pH 8.0. This short half-life means that aqueous solutions of PMSF can be safely discarded after they have been rendered alkaline (pH >8.6) and stored for several hours at room temperature.
Materials
MATERIALS
PMSFP212121Catalog #RP-P20270
STEP MATERIALS
PMSFP212121Catalog #RP-P20270
DMSOP212121Catalog #GB-D-360
PMSFP212121Catalog #RP-P20270
PMSFP212121Catalog #RP-P20270
DMSOP212121Catalog #GB-D-360
PMSFP212121Catalog #RP-P20270
Protocol materials
PMSFP212121Catalog #RP-P20270
DMSOP212121Catalog #GB-D-360
Troubleshooting
Weigh 1.74 g PMSF
2 µL
00:03:00
PMSFP212121Catalog #RP-P20270
Add DMSO to a final volume of 100 ml.
Dissolve completely.
00:03:00
DMSOP212121Catalog #GB-D-360
Stocks may be kept at -20°C for up to 6 months.
PMSF is used at a final concentration of 0.1- 1.0 mM
PMSFP212121Catalog #RP-P20270