Nov 01, 2014

Public workspaceHow to Make a 0.5M TCEP Stock Solution

DOI
dx.doi.org/10.17504/protocols.io.cizuf5
Sean Seaver
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DOI: dx.doi.org/10.17504/protocols.io.cizuf5
External link: http://store.p212121.com/TCEP-HCl/
Protocol Citation: Sean Seaver: How to Make a 0.5M TCEP Stock Solution. protocols.io https://dx.doi.org/10.17504/protocols.io.cizuf5
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: October 23, 2014
Last Modified: March 28, 2018
Protocol Integer ID: 313
Abstract
How to Make a 0.5M TCEP Stock Solution
Guidelines
TCEP can be dissolved in many types of aqueous buffers, and at a wide range of pH levels and still maintain stability

This allows you to prepare TCEP working concentrations and 10X stock solutions in the buffer of your choice before use.While TCEP can be used in Phosphate buffers, it has the tendency to be unstable around neutral pH levels.

If the TCEP is needed in the buffer, prepare it immediately before use.

A 50 mM TCEP solution may be substituted for DTT or β-Mercaptoethanol (β-ME) in SDS-PAGE loading buffers. TCEP cannot be used for isoelectric focusing, due to its charge in solution.
Materials
MATERIALS
ReagentTCEP HClP212121Catalog #SV-TCEP
STEP MATERIALS
ReagentTCEP HClP212121Catalog #SV-TCEP
ReagentTCEP HClP212121Catalog #SV-TCEP
ReagentTCEP HClP212121Catalog #SV-TCEP
ReagentTCEP HClP212121Catalog #SV-TCEP
Protocol materials
ReagentTCEP HClP212121Catalog #SV-TCEP
ReagentTCEP HClP212121Catalog #SV-TCEP
ReagentTCEP HClP212121Catalog #SV-TCEP
ReagentTCEP HClP212121Catalog #SV-TCEP
ReagentTCEP HClP212121Catalog #SV-TCEP
ReagentTCEP HClP212121Catalog #SV-TCEP
ReagentTCEP HClP212121Catalog #SV-TCEP
Weigh 5.73 g of TCEP
ReagentTCEP HClP212121Catalog #SV-TCEP
Add 35 ml of cold molecular biology grade water to the vial, and dissolve the TCEP. This resulting solution is very acidic, with an approximate pH of 2.5.
Duration00:03:00
ReagentTCEP HClP212121Catalog #SV-TCEP
Bring the solution to pH 7.0 with 10 N NaOH or 10 N KOH.
Bring the resulting solution to 40 ml with molecular biology grade water.
Aliquot into 1 ml volumes in freezer tubes and store at -20°C.