This protocol is designed for high sensitivity miRNA library generation for the Illumina sequencing platform. Our protocol and the enhanced reagent kit enables the discovery and profiling of small RNAs from a variety of sources including FFPE, exosome, serum, and whole blood. The TailorMix workflow is designed for ease of use, enabling library preparation a single day.User Friendly Workflow: Libraries can be prepared in a single day.No Additional Reagents Necessary: All reaction enzymes and buffers are provided.Stress Free Gel Purification: TailorCut Gel Extraction Tool Set is included for easy gel excision and purification.Easy to Use: Reagents are supplied as ready-to-use mixtures which improves consistency and reproducibility.Ultra High Sensitivity: Prepare miRNA libraries from any source with as little as 10ng of total RNA input.