This protocol is a minor modification of that reported by Culley and Steward (2007). As the starting point for this protocol, we assume that viruses have been collected on an aluminum\u00a0oxide 0.02-\u00b5m syringe-tip filter (Anotop, Whatman), but other filters capable of capturing viruses may be substituted.This is a protocol from:\u00a0Steward, G. F. and A. I. Culley. 2010. Chapter 16: Extraction and purification of nucleic acids from viruses.\u00a0Manual of Aquatic Viral Ecology. Waco, TX:American Society of Limnology and Oceanography. doi:10.4319\/mave.2010.978-0-9845591-0-7Please see the published manuscript for additional information.