The following is a modification of a Scherz R., 2001 method for colony embedding. The protocol described here is from:Sarah Piccirillo, et. al. The Rim101p/PacC Pathway and Alkaline pH Regulate Pattern Formation in Yeast Colonies(2010)Genetics184:707-716; doi:10.1534/genetics.109.113480Please see the full manuscript for additional details.