Jun 17, 2015

Public workspaceEGTA tetrasodium salt

DOI
dx.doi.org/10.17504/protocols.io.c3pymm
  • Matthew Sullivan1
  • 1Matthew Sullivan Lab, University of Arizona/Ohio State University
  • VERVE Net
  • Sullivan Lab
Bonnie Poulos
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DOI: dx.doi.org/10.17504/protocols.io.c3pymm
Protocol Citation: Matthew Sullivan 2015. EGTA tetrasodium salt. protocols.io https://dx.doi.org/10.17504/protocols.io.c3pymm
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: June 17, 2015
Last Modified: January 19, 2018
Protocol Integer ID: 847
Abstract
For use in DNase I Treatment protocol for DNase inactivation
Guidelines
Note: EGTA tetratsodium salt is far easier to get into solution than the disodium salt. It is possible to get to 1.5M with this reagent.
Materials
MATERIALS
ReagentEGTACatalog #E8145
STEP MATERIALS
ReagentEGTACatalog #E8145
ReagentEGTACatalog #E8145
Protocol materials
ReagentEGTACatalog #E8145
ReagentEGTACatalog #E8145
ReagentEGTACatalog #E8145
ReagentEGTACatalog #E8145
Weigh out 4.68 g and place in 5 ml molecular biology grade water.
Amount5 g
ReagentEGTACatalog #E8145
Stir until dissolved.
Measure volume and determine molarity: 1M = 4.68g/10 ml, therefore, measured volume/10 ml = final molarity.