Drosophila melanogaster females' productive system has been an excellent model in studying many developmental events such as stem cell specification and cell migration. The ovaries are composed of a chain of egg chambers starting with the germaruim that buds of stage 1 egg chamber after encapsulated it with follicle cells. Then, stage 1 egg chamber goes under 14 developmental stages. This protocol provides the basic method for both dissecting and immunostaining ovaries. Each step is critical. This protocol is modified from http://www.ncbi.nlm.nih.gov/pubmed/8162854