This protocol is from:Hans-W. Ackermann and Mikal Heldal. 2010. Chapter 18: Basic electron microscopy of aquatic viruses. Manual of Aquatic Viral Ecology. Waco, TX:American Society of Limnology and Oceanography. doi:10.4319/mave.2010.978-0-9845591-0-7 Please see the full manuscript for additional details.