Feb 02, 2016
BG-11 Medium for Freshwater Cyanobacteria
- Li Deng1
- 1Matthew Sullivan Lab, University of Arizona, Ohio State University
- VERVE Net
- Sullivan Lab
Protocol Citation: Li Deng 2016. BG-11 Medium for Freshwater Cyanobacteria. protocols.io https://dx.doi.org/10.17504/protocols.io.ddd225
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: July 13, 2015
Last Modified: March 21, 2018
Protocol Integer ID: 1157
Keywords: medium for freshwater cyanobacteria, freshwater cyanobacteria, cyanobacteria, most cyanobacteria, vitamin b12
Abstract
This medium is used successfully for most cyanobacteria. Vitamin B12 may be added for those species that require it. Use f/2 vitamin solution.
Guidelines
| STOCK | STOCK SOLUTION | ml/Litre | |
| NaNO3 | 150 g/L | 10 ml | |
| K2HPO4·3H2O or *K2HPO4 | 40 g/L or *30 g/L | 1 ml | |
| MgSO4·7H2O | 75 g/L | 1 ml | |
| CaCl2·2H2O | 36 g/L | 1 ml | |
| Citric Acid combined with Ferric Ammonium Citrate | 6 g/L | 1 ml | |
| EDTA | 1 g/L | 1 ml | |
| Na2CO3 | 20 g/L | 1 ml | |
| Trace Metal Solution | See below | 1 ml |
Adjust pH to approximately 7.5. (Initial pH is approximately 8.5.) When making solid media, you can add agar directly to medium or make double strength medium and double strength agar solution, then after autoclaving combine the two. OPTION: 0.5 g/L of HEPES buffer can be added to the final medium as a buffer. FeCl3 and EDTA added in a 1:1 ratio may be substituted.
Trace Metal Solution:
| Substance | g/Litre | |
| H3BO3 | 2.86 g | |
| MnCl2·4H2O | 1.81 g | |
| ZnSO4·7H2O | 0.222 g | |
| NaMoO4·5H2O | 0.390 g | |
| CuSO4·5H2O | 0.079 g | |
| Co(NO3)2·6H2O | 0.0494 g |
Dissolve each of the above substances separately prior to adding the next on the list.
Rippka, R., J. Deruelles, J. Waterbury, M. Herdman and R. Stanier. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111: 1-61
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