Mar 12, 2026

50% v/v Sterile Glycerol  V.1

This  protocol  is a draft, published without a DOI.
  • Tamra Lahom1
  • 1Carleton College
Tamra Mbeuh Lahom Lot
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Protocol Citation: Tamra Lahom 2026. 50% v/v Sterile Glycerol . protocols.io https://dx.doi.org/
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 12, 2026
Last Modified: March 12, 2026
Protocol  Integer ID: 313124
Keywords: term glycerol storage, bacteria, optimal concentration
Abstract
The optimal concentration of long-term glycerol storage is unknown. Most labs store bacteria in 15-25% glycerol.
Collect Glassware
Collect a 0.250 L bottle with top.

Dilute Glycerol
Pour 1 part (50 mL) of glycerol with 1 part (50 mL) of deionized H2O
Autoclave
Place autoclave tape on the lid of the bottle and autoclave at 121°C for 20-30 minutes
Note
Make sure the lid is loosened to avoid pressure build up.

Storage
Store at room temperature.