Mar 12, 2026
Version 1
50% v/v Sterile Glycerol V.1
This protocol is a draft, published without a DOI.
- Tamra Lahom1
- 1Carleton College
Protocol Citation: Tamra Lahom 2026. 50% v/v Sterile Glycerol . protocols.io https://protocols.io/view/50-v-v-sterile-glycerol-jv2ccn8ax
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 12, 2026
Last Modified: March 12, 2026
Protocol Integer ID: 313124
Keywords: term glycerol storage, bacteria, optimal concentration
Abstract
The optimal concentration of long-term glycerol storage is unknown. Most labs store bacteria in 15-25% glycerol.
Troubleshooting
Collect Glassware
Collect a 0.250 L bottle with top.
Dilute Glycerol
Pour 1 part (50 mL) of glycerol with 1 part (50 mL) of deionized H2O
Autoclave
Place autoclave tape on the lid of the bottle and autoclave at 121°C for 20-30 minutes
Note
Make sure the lid is loosened to avoid pressure build up.
Storage
Store at room temperature.