Jun 03, 2020
Version 2
50 mM Phosphate Buffer (with 0.02% sodium azide option) V.2
- Allen Institute for Brain Science1
- 1Allen Institute
- BICCN / BICAN
- Allen Institute for Brain Science
Protocol Citation: Allen Institute for Brain Science 2020. 50 mM Phosphate Buffer (with 0.02% sodium azide option). protocols.io https://dx.doi.org/10.17504/protocols.io.bg52jy8e
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 03, 2020
Last Modified: March 17, 2021
Protocol Integer ID: 37786
Keywords: RP0197, tissuecyte, buffer, embedding, brain, mouse,
Abstract
50 mM Phosphate Buffer can be used as a general buffer solution for multiple applications. It is suitable for use as a buffer for specimen embedding, as part of brain preparation associated with TissueCyte 2P Serial Imaging, described in PF0315 TissueCyte Specimen Embedding Acrylamide Coembedding.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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