May 14, 2024

Public workspace4% PFA + 2.5% Glutaraldehyde Fixative V.3

  • Allen Institute for Brain Science1
  • 1Allen Institute
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Protocol CitationAllen Institute for Brain Science 2024. 4% PFA + 2.5% Glutaraldehyde Fixative. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gpr15dvzp/v3Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 03, 2020
Last Modified: May 14, 2024
Protocol Integer ID: 99811
Keywords: RP0229, tissue, electrophysiology, reagent preparation,
Abstract
This protocol is used to prepare 4% Paraformaldehyde (PFA) + 2.5% Glutaraldehyde Fixative. This is utilized to fix the tissue after electrophysiology recordings.

Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.