May 20, 2020
2100 Bioanalyzer Operation for PCR, GTP and IVT Products
- Allen Institute for Brain Science1
- 1Allen Institute
- BICCN / BICAN
- Allen Institute for Brain Science
Protocol Citation: Allen Institute for Brain Science 2020. 2100 Bioanalyzer Operation for PCR, GTP and IVT Products. protocols.io https://dx.doi.org/10.17504/protocols.io.bfzjjp4n
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 06, 2020
Last Modified: March 17, 2021
Protocol Integer ID: 36619
Keywords: MB0004, polymerase chain reaction, genotyping, in vitro transcription, QC,
Abstract
This protocol describes bioanalyzer operation. The Agilent Bioanalyzer is used as a quality control step for PCR and IVT products. Peak size, shape, and sample concentration are recorded and used to evaluate samples on a pass/fail level.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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