Oct 16, 2020

Public workspace1M KPO4 pH6.0

DOI
dx.doi.org/10.17504/protocols.io.bnh2mb8e
  • 1Imperial College London
  • Behavioural Genomics
Ida Barlow
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DOI: dx.doi.org/10.17504/protocols.io.bnh2mb8e
Protocol Citation: Ida Barlow 2020. 1M KPO4 pH6.0. protocols.io https://dx.doi.org/10.17504/protocols.io.bnh2mb8e
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 16, 2020
Last Modified: October 16, 2020
Protocol Integer ID: 43290
Abstract
Protocol for making phosphate salt solution for adding to NGM
Materials
MATERIALS
ReagentPotassium phosphate (dibasic)P212121
ReagentPotassium phosphate (monobasic)P212121
ReagentPotassium hydroxideP212121
Make 1M solutions of monobasic and dibasic salts
Make 1M solutions of monobasic and dibasic salts
Prepare 1M solution dibasic salt:

Amount174.18 g K2HPO4
Amount1 L water

Prepare 1M solutions of monobasic salt:

Amount136.09 g KH2PO4
Amount1 L water

Autoclave both solutions and allow to cool

Mix salts to achieve correct pH
Mix salts to achieve correct pH
Mix prepared salt solutions to achieve 1M KPO4 solution close to pH6:

Amount100 mL 1M K2HPO4 (dibasic)
Amount400 mL 1M KH2PO4 (monobasic)


Adjust pH to phH6.0 using pH meter and addition of drops of KOH