Feb 01, 2024

12S rRNA Environmental DNA Processing Pipeline with size selection

This collection is a draft, published without a DOI.
  • 1Monterey Bay Aquarium Research Institution
  • Better Biomolecular Ocean Practices (BeBOP)
Kathleen Pitz
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Collection Citation: Kathleen Pitz, Jacoby Baker 2024. 12S rRNA Environmental DNA Processing Pipeline with size selection. protocols.io https://dx.doi.org/
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this collection
Created: January 30, 2024
Last Modified: February 01, 2024
Collection  Integer ID: 94367
Keywords: rrna environmental dna processing pipeline, rrna gene, bony fish in seawater, environmental dna sample, targeting bony fish, seawater, dna, sequencing
Abstract
Protocol chain from extraction to sequencing for amplification of the 12S rRNA gene targeting bony fish in seawater environmental DNA samples.
Files
Protocol
Name
Environmental DNA (eDNA) extraction using Qiagen DNeasy 96 Blood and Tissue Kit
Version 2
Created by
Image of Kathleen Pitz, Monterey Bay Aquarium Research Institute
Kathleen PitzMonterey Bay Aquarium Research Institute
Protocol
Name
Environmental DNA (eDNA) 12S Metabarcoding PCR Protocol (with Platinum SuperFi II Taq)
Version 2
Created by
Image of Kathleen Pitz, Monterey Bay Aquarium Research Institute
Kathleen PitzMonterey Bay Aquarium Research Institute
Protocol
Name
Environmental DNA (eDNA) 12S Metabarcoding Illumina MiSeq NGS Protocol with size selection
Version 2
Created by
Image of Kathleen Pitz, Monterey Bay Aquarium Research Institute
Kathleen PitzMonterey Bay Aquarium Research Institute