Oct 30, 2024
10xV3.1 HT CellPlex Genomics Sample Processing Protocol
- Allen Institute1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute 2024. 10xV3.1 HT CellPlex Genomics Sample Processing Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.8epv5269dv1b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 30, 2024
Last Modified: October 30, 2024
Protocol Integer ID: 111277
Keywords: 10xHT, CellPlex, ht cellplex genomics sample processing protocol this protocol, ht cellplex genomics sample processing protocol, genomics sample processing protocol this protocol, genomics sample processing protocol, multiplexed cell, multiplexed sample, ht protocol, feature barcode technology, cell, utilizing feature barcode technology
Abstract
This protocol is used for rapid generation of 3’ transcriptomic-NGS-ready- single-cell-libraries from pools of multiplexed cells by utilizing Feature Barcode technology and doubling the capacity of the original 10xV3.1 HT protocol, allowing for a capture of ~40,000 multiplexed samples instead of ~20,000 cells from a single sample per port.
Troubleshooting