Aug 11, 2023

Public workspace10x Protocols: Visium v2 CytAssist FFPE Deparaffinization, H&E staining, and Imaging-- University of Minnesota TMCs (CG000520 Rev B)

DOI
https://dx.doi.org/10.17504/protocols.io.dm6gpjpyjgzp/v1
  • IOx Genomics1,
  • Laura J Niedernhofer2,
  • David A Bernlohr2
  • Cellular Senescence Network (SenNet) Method Development Community
Mickayla DuFresne-To
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DOI: https://dx.doi.org/10.17504/protocols.io.dm6gpjpyjgzp/v1
Protocol Citation: IOx Genomics, Laura J Niedernhofer, David A Bernlohr 2023. 10x Protocols: Visium v2 CytAssist FFPE Deparaffinization, H&E staining, and Imaging-- University of Minnesota TMCs (CG000520 Rev B). protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gpjpyjgzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 05, 2023
Last Modified: August 11, 2023
Protocol Integer ID: 81461
Keywords: UMN, 10x, Visium, CytAssist, FFPE, Spatial, UMinnesota, University of Minnesota, Niedernhofer, Bernlohr, protocols from 10x genomic, visium v2 cytassist ffpe deparaffinization, 10x genomic, visium spatial gene expression, visium spatial gene expression v2 chemistry, 10x genomics support website, 10x protocol cg000520, 10x protocol, cytassist component, genomic, gene expression
Funders Acknowledgements:
NIH
Grant ID: 1U54AG07041-01
NIH
Grant ID: 1U54AG079754-01
Abstract
Protocols from 10x Genomics for Visium Spatial Gene Expression v2 chemistry on FPPE samples with the CytAssist component.

Protocol ID# (CG) and Revision used:
10x protocol CG000520, Revision B -- Deparaffinization, H&E staining, and Imaging prior to Library construction with CytAssist.

Note: These protocols may not be the current version offered by 10x Genomics but were used to produce the specific datasets connected to them. Please review the 10x Genomics support website for the most recent versions of the protocols prior to starting your experiment.
Troubleshooting
10x protocol CG000520, Revision B (Deparaffinization, H&E staining and Imaging prior to Library Construction and CytAssist)
Download 10x CytAssist Visium Deparaffin HE, Rev B (Protocol CG000520).pdf10x CytAssist Visium Deparaffin HE, Rev B (Protocol CG000520).pdf