Aug 11, 2023

Public workspace10x Protocols: Visium v1 FFPE Deparaffinization, H&E staining, and Imaging -- University of Minnesota TMCs (CG000409 Rev B)

DOI
dx.doi.org/10.17504/protocols.io.5qpvorm3bv4o/v1
  • IOx Genomics1,
  • Laura J Niedernhofer2,
  • David A Bernlohr2
  • 110x Genomics, info@10xgenomics.com;
  • 2University of Minnesota, Minneapolis, MN USA
  • Cellular Senescence Network (SenNet) Method Development Community
Mickayla DuFresne-To
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DOI: dx.doi.org/10.17504/protocols.io.5qpvorm3bv4o/v1
Protocol Citation: IOx Genomics, Laura J Niedernhofer, David A Bernlohr 2023. 10x Protocols: Visium v1 FFPE Deparaffinization, H&E staining, and Imaging -- University of Minnesota TMCs (CG000409 Rev B). protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvorm3bv4o/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 03, 2023
Last Modified: August 11, 2023
Protocol Integer ID: 81364
Keywords: 10x, Spatial, Visium, FFPE, UMN, UMinnesota, University of Minnesota, Niedernhofer, Bernlohr
Funders Acknowledgements:
NIH
Grant ID: 1U54AG07041-01
NIH
Grant ID: 1U54AG079754-01
Abstract
Protocols from 10x Genomics for Visium Spatial Gene Expression v1 chemistry on FPPE samples without the CytAssist component.

Protocol ID# (CG) and Revision letter provided here:
10x protocol CG000409, Revision B -- Deparaffinization, H&E staining, coverslipping, and imaging

Additional protocols added for guidance:
10x protocol for imaging guidelines
10x protocol for tissue preparation guidelines

Note: These protocols may not be the current version offered by 10x Genomics but were used to produce the specific datasets connected to them. Please review the 10x Genomics support website for the most recent versions of the protocols prior to starting your experiment.
10x protocol CG000409, Rev B (Deparaffinization, H&E, etc)
Download CG000409_Demonstrated_Protocol_VisiumSpatialFFPE_Deparaffin_H&E_RevB.pdfCG000409_Demonstrated_Protocol_VisiumSpatialFFPE_Deparaffin_H&E_RevB.pdf

Additional Protocols/Resources
Download 10x Manual Visium FFPE Imaging Guidelines, Rev A (Protocol CG000436).pdf10x Manual Visium FFPE Imaging Guidelines, Rev A (Protocol CG000436).pdf
Download 10x Manual Visium Spatial FFPE Tissue Preparation Guide.pdf10x Manual Visium Spatial FFPE Tissue Preparation Guide.pdf
https://www.10xgenomics.com/support/spatial-gene-expression-ffpe