Aug 11, 2023
10x Protocols: Visium Fresh Frozen Methanol Fixation, H&E Staining & Imaging-- University of Minnesota TMCs (CG000160 Rev C)
- 10x Genomics1,
- Laura J Niedernhofer2,
- David A Bernlohr2
- 110x Genomics, [email protected];
- 2University of Minnesota, Minneapolis, MN USA
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: 10x Genomics, Laura J Niedernhofer, David A Bernlohr 2023. 10x Protocols: Visium Fresh Frozen Methanol Fixation, H&E Staining & Imaging-- University of Minnesota TMCs (CG000160 Rev C). protocols.io https://dx.doi.org/10.17504/protocols.io.kxygx96nkg8j/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 05, 2023
Last Modified: August 11, 2023
Protocol Integer ID: 81456
Keywords: Fresh Frozen, OCT, UMN, 10x Genomics, Visium, UMinnesota, University of Minnesota, Human, Spatial, protocols from 10x genomic, 10x protocol for tissue preparation guideline, visium spatial gene expression on fresh frozen, 10x genomic, visium spatial gene expression, 10x genomics support website, 10x protocol for imaging guideline, visium fresh frozen methanol fixation, tissue preparation guideline, 10x protocol, genomic, imaging guideline, methanol fixation, gene expression, 10x, cg000240
Funders Acknowledgements:
NIH
Grant ID: 1U54AG07041-01
NIH
Grant ID: 1U54AG079754-01
Abstract
Protocols from 10x Genomics for Visium Spatial Gene Expression on Fresh Frozen / OCT samples. Completed without CytAssist component.
Version of protocol used:
10x protocol CG000160 Revision C -- Methanol Fixation, H&E staining, and imaging
Additional protocols added for guidance:
10x protocol for imaging guidelines (CG000241, Rev D)
10x protocol for tissue preparation guidelines (CG000240, Rev D)
Note: These protocols may not be the current version offered by 10x Genomics but were used to produce the specific datasets connected to them. Please review the 10x Genomics support website for the most recent versions of the protocols prior to starting your experiment.
Troubleshooting
10x protocol CG000160, Rev C (Fixation/H&E etc):
10x FF Visium Methanol Fixation and HE Staining.pdf Additional Protocols/Resources
10x FF Visium Imaging Guidelines .pdf CG000241, Rev D10x FF Visium Spatial Tissue Preparation Guide.pdf CG000240, Rev D