Aug 11, 2023
10x Protocols: Visium Fresh Frozen Library Construction -- University of Minnesota TMCs (CG000239 Rev F)
- 10x Genomics1,
- Laura J Niedernhofer2,
- David A Bernlohr2
- 110x Genomics, [email protected];
- 2University of Minnesota, Minneapolis, MN USA
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: 10x Genomics, Laura J Niedernhofer, David A Bernlohr 2023. 10x Protocols: Visium Fresh Frozen Library Construction -- University of Minnesota TMCs (CG000239 Rev F). protocols.io https://dx.doi.org/10.17504/protocols.io.81wgby341vpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 05, 2023
Last Modified: August 11, 2023
Protocol Integer ID: 81457
Keywords: Visium, Fresh Frozen, Library construction, 10x Genomics, UMN, UMinnesota, University of Minnesota, OCT, Spatial, protocols from 10x genomic, 10x genomic, visium spatial gene expression, 10x genomics support website, visium spatial gene expression on fresh frozen, 10x protocol for tissue preparation guideline, 10x protocol for imaging guideline, 10x protocol cg000239, 10x protocol, genomic, tissue preparation guideline, gene expression, 10x, visium fresh frozen library construction, recent versions of the protocol
Funders Acknowledgements:
NIH
Grant ID: 1U54AG07041-01
NIH
Grant ID: 1U54AG079754-01
Abstract
Protocols from 10x Genomics for Visium Spatial Gene Expression on Fresh Frozen / OCT samples.
Completed without CytAssist component.
Version of protocol used:
10x protocol CG000239, Revision F -- Library Construction
Additional protocols added for guidance:
10x protocol for imaging guidelines (CG000241, Rev D)
10x protocol for tissue preparation guidelines (CG000240, Rev D)
Note: These protocols may not be the current version offered by 10x Genomics but were used to produce the specific datasets connected to them. Please review the 10x Genomics support website for the most recent versions of the protocols prior to starting your experiment.
Troubleshooting
10x protocol CG000239, Rev F (Library construction):
10x FF Visium Spatial Gene Expression User Guide.pdf Additional Protocols/Guidelines
10x FF Visium Imaging Guidelines .pdf CG000241, Rev D10x FF Visium Spatial Tissue Preparation Guide.pdf CG000240, Rev D