Jun 26, 2023

10x Protocols: Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling -- University of Minnesota TMCs (CG000553 Rev A)

DOI
https://dx.doi.org/10.17504/protocols.io.q26g7y2pkgwz/v1
  • IOx Genomics1,
  • Laura Niedernhofer2
  • 110x Genomics, [email protected];
  • 2University of Minnesota Medical School, Minneapolis, MN USA
  • Cellular Senescence Network (SenNet) Method Development Community
Mickayla DuFresne-To
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DOI: https://dx.doi.org/10.17504/protocols.io.q26g7y2pkgwz/v1
Protocol Citation: IOx Genomics, Laura Niedernhofer 2023. 10x Protocols: Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling -- University of Minnesota TMCs (CG000553 Rev A). protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7y2pkgwz/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 03, 2023
Last Modified: June 26, 2023
Protocol Integer ID: 81379
Keywords: 10x, scRNAseq, Fixed, Dissociation, Fix-Dissociation, UMN, UMinnesota, University of Minnesota, preparation for 10x chromium flex scrnaseq, chromium fixed rna profiling, dissociation for chromium fixed rna profiling, 10x chromium flex scrnaseq, 10x genomics protocol, preparation for chromium single cell expression, 10x genomic, chromium single cell expression, chromium single cell, 10x protocol cg000553, tissue fixation, 10x protocol, rna, genomic, dissociation of whole tissue, whole tissue, single cell, 10x
Abstract
10x Genomics protocol where you fix the whole tissue and then dissociate it in preparation for Chromium Single Cell Expression flex

Protocol ID# (CG) and Revision letter provided here:
10x protocol CG000553, Revision A-- Fixation and dissociation of whole tissue; preparation for 10x Chromium Flex scRNAseq

Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.
Download 10x Tissue Fixation+Dissociation, Rev A (Protocol CG000553).pdf10x Tissue Fixation+Dissociation, Rev A (Protocol CG000553).pdf