Mar 22, 2024

10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Multiplex -- University of Minnesota TMCs (CG000527 Rev E) V.2

DOI
https://dx.doi.org/10.17504/protocols.io.bp2l6x81klqe/v2
  • IOx Genomics1,
  • Laura Niedernhofer2,
  • David A Bernlohr2
  • Cellular Senescence Network (SenNet) Method Development Community
Allie Abdellatif
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DOI: https://dx.doi.org/10.17504/protocols.io.bp2l6x81klqe/v2
Protocol Citation: IOx Genomics, Laura Niedernhofer, David A Bernlohr 2024. 10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Multiplex -- University of Minnesota TMCs (CG000527 Rev E). protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l6x81klqe/v2Version created by Allie Abdellatif
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 22, 2024
Last Modified: March 22, 2024
Protocol  Integer ID: 97156
Keywords: 10x genomics chromium single cell expression, nuclei gene expression flex multiplex, 10x genomic, chromium single cell, flex protocol, 10x protocol, gene expression, cell, recent versions of the protocol, protocol, nuclei, chromium
Funders Acknowledgements:
NIH
Grant ID: 5U54AG076041-03
NIH
Grant ID: 5U54AG079754-02
Abstract
10x Genomics Chromium Single Cell Expression flex protocol for library construction.

Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.
Preparation
Complete single cell or nuclei isolation and 10x fixation prior to starting this protocol
Library Preparation
Download CG000315_Rev-E.pdfCG000315_Rev-E.pdf4.2MB
Note
 Sequence with the read format 29,10,10,89

FASTQ Generation
BCL data from Illumina sequencer is demultiplexed and converted into FASTQ format using bcl2fastq version 2.20.0