Aug 11, 2023

Public workspace10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Fixation -- University of Minnesota TMCs (CG000478 Rev B)

DOI
https://dx.doi.org/10.17504/protocols.io.81wgby3x3vpk/v1
  • IOx Genomics1,
  • Laura J Niedernhofer2,
  • David A Bernlohr2
  • Cellular Senescence Network (SenNet) Method Development Community
Mickayla DuFresne-To
Icon indicating open access to content
QR code linking to this content
DOI: https://dx.doi.org/10.17504/protocols.io.81wgby3x3vpk/v1
Protocol Citation: IOx Genomics, Laura J Niedernhofer, David A Bernlohr 2023. 10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Fixation -- University of Minnesota TMCs (CG000478 Rev B). protocols.io https://dx.doi.org/10.17504/protocols.io.81wgby3x3vpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 03, 2023
Last Modified: August 11, 2023
Protocol Integer ID: 81381
Keywords: 10x, scRNAseq, snRNAseq, sc/snRNAseq, Fixed, UMN, 10x genomics fixation for chromium single cell expression, 10x genomics chromium flex protocol, applying 10x genomics chromium, 10x genomics fixation, nuclei gene expression flex fixation, chromium single cell expression, chromium single cell, 10x genomic, fixation of single cell, single cell, gene expression, flex protocol, dois for dissociation protocol, 10x protocol, genomic, 10x cg000478, nuclei, gene, cell, dissociation protocol
Funders Acknowledgements:
NIH
Grant ID: 1U54AG07041-01
NIH
Grant ID: 1U54AG079754-01
Abstract
DOIs for dissociation protocols and 10x Genomics fixation for Chromium Single Cell Expression flex protocols.

Please see DOIs for dissociation protocols linked here.

Protocol ID# (CG) and Revision letter provided here:
10x CG000478, Revision B -- Fixation of single cells and nuclei prior to applying 10x Genomics Chromium flex protocols.

Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.
10x Protocol CG000478, Rev B (Fixation):
Download 10x Cell+Nuclei Fixation Chromium Fixed RNA Profiling, Rev B (Protocol CG000478).pdf10x Cell+Nuclei Fixation Chromium Fixed RNA Profiling, Rev B (Protocol CG000478).pdf