Jun 26, 2023

10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Fixation for sc/sn -- University of Minnesota TMCs (CG000478 Rev A)

DOI
https://dx.doi.org/10.17504/protocols.io.ewov1opq2lr2/v1
  • IOx Genomics1,
  • Laura Niedernhofer2
  • 110x Genomics, [email protected];
  • 2University of Minnesota Medical School, Minneapolis, MN, USA
  • Cellular Senescence Network (SenNet) Method Development Community
Mickayla DuFresne-To
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DOI: https://dx.doi.org/10.17504/protocols.io.ewov1opq2lr2/v1
Protocol Citation: IOx Genomics, Laura Niedernhofer 2023. 10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Fixation for sc/sn -- University of Minnesota TMCs (CG000478 Rev A). protocols.io https://dx.doi.org/10.17504/protocols.io.ewov1opq2lr2/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 03, 2023
Last Modified: June 26, 2023
Protocol  Integer ID: 81369
Keywords: 10x, scRNAseq, snRNAseq, fixation, UMN, UMinnesota, University of Minnesota, 10x genomics fixation for chromium single cell expression, 10x genomics chromium flex protocol, applying 10x genomics chromium, 10x genomics fixation, nuclei gene expression flex fixation, chromium single cell expression, chromium single cell, 10x genomic, fixation of single cell, gene expression, single cell, flex protocol, genomic, protocol for fixation, 10x protocol, dois for dissociation protocol, gene, nuclei, 10x cg000478, cell
Abstract
DOIs for dissociation protocols and 10x Genomics fixation for Chromium Single Cell Expression flex protocols.

Protocol ID# (CG) and Revision letter provided here:
10x CG000478, Revision A -- Protocol for fixation of single cells and nuclei prior to applying 10x Genomics Chromium flex protocols.

Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.
10x Protocol CG000478, Rev A (Fixation):
Download 10x Fixation of Cells and Nuclei (CG000478, Rev A).pdf10x Fixation of Cells and Nuclei (CG000478, Rev A).pdf