Jun 26, 2023

10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Multiplex-- University of Minnesota TMCs (CG000527 Rev C)

DOI
https://dx.doi.org/10.17504/protocols.io.5jyl8jy4dg2w/v1
  • IOx Genomics1,
  • Laura Niedernhofer2
  • 110x Genomics, [email protected];
  • 2University of Minnesota Medical School, Minneapolis, MN, USA
  • Cellular Senescence Network (SenNet) Method Development Community
Mickayla DuFresne-To
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DOI: https://dx.doi.org/10.17504/protocols.io.5jyl8jy4dg2w/v1
Protocol Citation: IOx Genomics, Laura Niedernhofer 2023. 10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Multiplex-- University of Minnesota TMCs (CG000527 Rev C). protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl8jy4dg2w/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 11, 2023
Last Modified: June 26, 2023
Protocol  Integer ID: 81752
Keywords: 10x, scRNAseq, snRNAseq, sc/snRNAseq, Fixed, UMN, University of Minnesota, UMinnesota, 10x genomics chromium single cell expression, 10x genomic, chromium single cell, 10x protocol, protocol cg000527, flex protocol, single cell, gene expression, library construction of 10x, genomic, recent versions of the protocol, gene, nuclei, protocol, 10x, cg
Abstract
10x Genomics Chromium Single Cell Expression flex protocol for library construction.

Protocol ID# (CG) and Revision letter provided here:
10x protocol CG000527, Revision C -- Library construction of 10x fixed single cell or nuclei

Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.
Complete single cell or nuclei isolation and 10x fixation prior to starting this protocol
10x Protocol CG000527, Revision C (Library Construction):
Download 10x Chromium Fixed RNA Profiling, Multiplexed Samples, Rev C (Protocol CG000527).pdf10x Chromium Fixed RNA Profiling, Multiplexed Samples, Rev C (Protocol CG000527).pdf