Mar 14, 2026
Version 4
10x Multiome Sample Processing V.4
- Allen Institute for Brain Science1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute for Brain Science 2026. 10x Multiome Sample Processing. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l61mqrvqe/v4Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 14, 2026
Last Modified: March 14, 2026
Protocol Integer ID: 313259
Keywords: open chromatin region, chromatin, 10x multiome sample processing protocol, single nuclei level, transposed nuclei, nuclei, national institutes of health
Abstract
Protocol is used to profile all the open chromatin regions at a single nuclei level through the rapid generation of NGS-ready libraries, and for rapid generation of 3' transcriptomic-NGS-ready-single-cell-libraries from the same pool of transposed nuclei.
"Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Numbers 1U01MH114812-01 and 1U19MH114830-01. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health".
