This protocol describes how to prepare a 100x M21x M2 media could be created from the stock and supplemental stocks and trace metals could be added afterwards.Recipes for standard and alternative M2 for culturing freshwater cyanobacteria, such as\u00a0Synechocystis sp. PCC 6803, in a co-culture togther with heterotrophic bacteria.Media is usually not suitable for marine cyanobacteria.Slighly modified from: Weiss, Taylor L., Eric J. Young, and Daniel C. Ducat. "A synthetic, light-driven consortium of cyanobacteria and heterotrophic bacteria enables stable polyhydroxybutyrate production." Metabolic engineering 44 (2017): 236-245.