Jun 07, 2024

Public workspace100ml 5M Sodium Chloride (NaCl)

This protocol is a draft, published without a DOI.
  • Menglin WANG1
  • 1UC Berkeley
  • California Insect Barcoding Initiatives
Menglin WANG
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Protocol Citation: Menglin WANG 2024. 100ml 5M Sodium Chloride (NaCl). protocols.io https://protocols.io/view/100ml-5m-sodium-chloride-nacl-dfa43igw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 07, 2024
Last Modified: June 07, 2024
Protocol Integer ID: 101436
Abstract
This is a recipe for how to make 100ml 5M Sodium Chloride (NaCl).
Protocol materials
ReagentSodium ChlorideFisher ScientificCatalog #S271
ReagentUltraPure Distilled Water Invitrogen - Thermo FisherCatalog #10977-015
ReagentSodium ChlorideFisher ScientificCatalog #S271
ReagentUltraPure Distilled Water Invitrogen - Thermo FisherCatalog #10977-015
5M Sodium Chloride (NaCl,100ml)
5M Sodium Chloride (NaCl,100ml)
20m
20m
Weigh out an amount of Amount29.22 g of ReagentSodium ChlorideFisher ScientificCatalog #S271 in an appropriate-size beaker or conical flask (1L volume). Add Amount80 mL of ReagentUltraPure Distilled Water Invitrogen - Thermo FisherCatalog #10977-015 .

Precaution: Do not add 100 ml of deionized/Milli-Q water. In most cases, solution volume increases when a large amount of solute is dissolved in a solvent.
Dissolve ReagentSodium ChlorideFisher ScientificCatalog #S271 using a magnetic stirrer or manual shaking with a glass stirring rod.

Tip: You can heat the solution to Temperature60 °C to accelerate the dissolution process.
Transfer the solution to a measuring cylinder or a volumetric flask and adjust the volume to Amount100 mL with ReagentUltraPure Distilled Water Invitrogen - Thermo FisherCatalog #10977-015 . Remix it.
Transfer the solution to an autoclavable bottle and sterilize it by autoclaving Duration00:20:00 at 15 lb/sq.in. (psi) from Temperature121-124 °C on liquid cycle.
20m
Storage: Store the solution at TemperatureRoom temperature .

Tip: NaCl may precipitate with time. Dissolve it by shaking the bottle vigorously.