May 15, 2024
Version 2
10% Tween 20 V.2
- Allen Institute for Brain Science1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute for Brain Science 2024. 10% Tween 20. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov18m7pgr2/v2Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 15, 2024
Last Modified: May 15, 2024
Protocol Integer ID: 99877
Keywords: RP0007, surfactant, immunohistochemistry, easier penetration of macromolecule, membrane, antisera, holes in membrane, macromolecule, cell, aqueous solution, organelle, surface tension of the solution,
Abstract
This protocol is used to prepare 10% Tween 20. Tween 20, when added to aqueous solutions acts as a surfactant, decreasing the surface tension of the solution. It also punches holes in membranes, allowing easier penetration of macromolecules into cells and organelles, and reduces non-specific binding of antisera in immunohistochemistry.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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