The protocol (~50 minutes) will run deparaffination with CitriSolv, rehydration with Ethanol, and washes with PBS.
a. 15 minute CitriSolv incubation.
b. 7.5 minute CitriSolv incubation.
c. 7.5 minute CitriSolv incubation.
d. 1 minute 100% Ethanol incubation.
e. 1 minute 100% Ethanol incubation.
f. 1 minute 70% Ethanol incubation.
g. 1 minute 50% Ethanol incubation.
h. 1 minute 30% Ethanol incubation.
During the run, pre-cool Singulator 200 for subsequent nuclei extraction from tissue.
Upon completion of the protocol, remove the cartridge from the machine.