Jul 07, 2019
05 Agarose Gel Electrophoresis
- 1Tianjin University
Protocol Citation: Tjusls China 2019. 05 Agarose Gel Electrophoresis. protocols.io https://dx.doi.org/10.17504/protocols.io.48rgzv6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 07, 2019
Last Modified: July 07, 2019
Protocol Integer ID: 25585
Guidelines
0 Mass Percent
Materials
MATERIALS
TAE (Tris-Acetate-EDTA) buffer, 1x
DNA samplesCatalog #/
1% Agarose gelCatalog #/
10×green loading bufferCatalog #/
Use 1×TAE buffer to prepare 1% Agarose mix in a flask, then put it in the microwave and heat it as long as it takes to completely dissolve the Agarose.
Take out the conical flask, cool it in the wash basin to about 50°C. Add EB quickly , and then mix well. Pour the Agarose gel into gel tray and insert comb into slots. Let the gel solidify for 15-20min. Meanwhile, dilute the 10x green buffer to 1x and add to the DNA samples.
50 °C
00:15:00 ~00:20:00
Place the gel onto the electrophoresis apparatus ensuring that it is totally submerged in 1xTAE buffer. Carefully load each sample into its designated lane and 2ul DNA marker into a separate lane.
2 µL
Run at 120V for 20-25 min. If the sample have not completely separated, the time may be extended appropriately.
00:15:00 ~00:20:00
Check the gel using a gel imager or under UV light, then take a photo oNorma.