Mar 17, 2018
- 1Dalhousie University
Protocol Citation: Angela Tsai 2018. 0.8% Agarose Gel. protocols.io https://dx.doi.org/10.17504/protocols.io.nv4de8w
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 17, 2018
Last Modified: March 28, 2018
Protocol Integer ID: 10908
Abstract
Purpose of this protocol is to create an agarose gel for visualizing and extracting DNA.
Add 100 mL of 1X TAE Buffer to 0.8 g of UltraPure Agarose and a few grains ofguanosine.
Microwave for 1 minute in conventional microwave, swirling at 30 seconds.
Allow to cool until it is not painful to touch and add 6 uL of Ethidium Bromide.
Pour into gel dock with comb and allow to solidify.