Mar 29, 2018

Public workspace0.5M EDTA

DOI
dx.doi.org/10.17504/protocols.io.n5idg4e
  • 1University of California, Riverside
Alex Rajewski
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DOI: dx.doi.org/10.17504/protocols.io.n5idg4e
External link: http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8030.full?text_only=true
Protocol Citation: Alex Rajewski 2018. 0.5M EDTA. protocols.io https://dx.doi.org/10.17504/protocols.io.n5idg4e
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 29, 2018
Last Modified: March 29, 2018
Protocol Integer ID: 11146
Abstract
This is a basic protocol to make a 0.5M EDTA stock solution at pH 8.0. I have mostly copied it from CSHL's recipe.
Materials
MATERIALS
ReagentNaOH
Reagentethylenediaminetetraacetic acid dihydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #E5134-100G
Safety warnings
The dissolution of NaOH pellets is an exothermic reaction, depending on the volumes it may become hot enough to burn/explode. This is unlikely but possible.
Add EDTA powder to an autoclavable, 500mL, screw-top bottle.
Amount93 g EDTA
Add ~400mL of DI water and stir with a magnetic stir bar. Note that the EDTA will not go into solution until the pH is adjusted in a later step.
Adjust the pH to 8.0 using NaOH pellets. It will take approximately 10g of pellets to adjust the pH this high. Add the pellets slowly wearing proper eye and skin protection. 
Amount10 g NaOH
Transfer the solution to a graduated cylinder, bring to 500mL volume with water, and return the solution to the screw-top bottle. Make sure to remove the stir bar when transferring the solution.
Autoclave the solution and store at room temperature.