Oct 12, 2015
0.5M EDTA
- Matthew Sullivan Lab1
- 1Matthew Sullivan Lab, University of Arizona, Ohio State University
- VERVE Net
- Sullivan Lab
External link: https://doi.org/10.1371/journal.pone.0249149
Protocol Citation: Matthew Sullivan Lab 2015. 0.5M EDTA. protocols.io https://dx.doi.org/10.17504/protocols.io.c58y9v
Manuscript citation:
Erster O, Shkedi O, Benedek G, Zilber E, Varkovitzky I, Shirazi R, Shorka DO, Cohen Y, Bar T, Yechieli R, Oikawa MT, Venkert D, Linial M, Oiknine-Djian E, Mandelboim M, Livneh Z, Shenhav-Saltzman G, Mendelson E, Wolf D, Szwarcwort-Cohen M, Mor O, Lewis Y, Zeevi D (2021) Improved sensitivity, safety, and rapidity of COVID-19 tests by replacing viral storage solution with lysis buffer. PLoS ONE 16(3): e0249149. doi: 10.1371/journal.pone.0249149
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: June 23, 2015
Last Modified: March 22, 2018
Protocol Integer ID: 928
Add 186.1 g EDTA disodium salt dihydrate (Mallinckrodt 4931) to 700 ml MilliQ water
Stir to dissolve
Bring pH to 8.0 with NaOH (~50ml of 10M NaOH or ~23g NaOH pellets)
Bring volume to 1L with MilliQ water
Autoclave