Feb 05, 2019

Public workspace0.5 M EDTA (0.5 L)

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DOI
dx.doi.org/10.17504/protocols.io.xr2fm8e
  • 1C.N.R.
Mattia Pegoraro
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DOI: dx.doi.org/10.17504/protocols.io.xr2fm8e
Protocol Citation: Mattia Pegoraro 2019. 0.5 M EDTA (0.5 L). protocols.io https://dx.doi.org/10.17504/protocols.io.xr2fm8e
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 05, 2019
Last Modified: February 05, 2019
Protocol Integer ID: 19994
Keywords: EDTA, TBE
Abstract
Is used in a multitude of experiments, but is often used as an ingredient in 10x TBE buffer
Materials
MATERIALS
ReagentEthylenediamine Tetraacetic Acid Disodium Salt DihydrateFisher ScientificCatalog #S311-500
ReagentSodium HydroxideFisher ScientificCatalog #BP359-500
Safety warnings
Will be using NaOH pellets, make sure to wear gloves.
Before start
Make sure you are able to use the pH machine and an autoclave machine
Fill 0.5 L beker with Amount300 mL of deionized water. 

Add Amount73.06 g of Ethylenediamine Tetraacetic Acid Disodium Salt (292,24 g/mol) to the water and mix.

Put the electrode pH meter into the solution (with continuos mixing) and mesure the pH (don't worry if the solution isn't clear). The pH will be about 3 at this point. The pH needs to be at 8. 
Add NaOH pellets until the pH is near 7.8 (the solution will be clear around pH 7.5). Complete to arrive at pH 8 with NaOH 5 M solution (also NaOH 10 M is good).
Once at pH 8, fill toAmount500 mL . 

Autoclave at Temperature121 °C for Duration00:15:00 or filter it with 0,22 u filter.

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