Dec 02, 2021

Public workspace0.05 M Glycine

DOI
dx.doi.org/10.17504/protocols.io.bybapsie
  • 1FDA
  • GenomeTrakr
    Tech. support email: genomeTrakr@fda.hhs.gov
Jessica L Jones
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.bybapsie
Protocol Citation: Jacquelina.Woods 2021. 0.05 M Glycine. protocols.io https://dx.doi.org/10.17504/protocols.io.bybapsie
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: September 16, 2021
Last Modified: December 02, 2021
Protocol Integer ID: 53314
Abstract
This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in wastewater​​. Protocols developed for this project cover wastewater collection, concentration, RNA extraction, RT-qPCR detection, library prep, genome sequencing, quality control checks, and data submission to NCBI. This method provides a reagent formula required in the rapid concentration of intact viruses from wastewater using a combination of PEG precipitation and ultracentrifugation.
Materials
ReagentGlycineSigma AldrichCatalog #G7126
Deionized or ultrapure water

Protocol materials
ReagentGlycineMerck MilliporeSigma (Sigma-Aldrich)Catalog #G7126
ReagentGlycineMerck MilliporeSigma (Sigma-Aldrich)Catalog #G7126
Mix Amount3.75 g ReagentGlycineSigma AldrichCatalog #G7126 in deionized or ultrapure water to Amount1 L

Adjust Ph9.5

Autoclave Temperature121 °C Duration00:15:00

15m
Store at Temperature2-8 °C